To further investigate whether M1 virus relies on host cell glycolysis to facilitate its replication, two glycolysis inhibitors—the glucose analog 2-DG and lonidamine, which inhibits hexokinase 2 (HK2), the first rate-limiting enzyme in glycolysis—were used to treat the colorectal carcinoma cell lines HCT 116 and HCT-8. This evidence concerns the gene HK2 and colorectal carcinoma.