The expression of gene for ACSL1 (Acsl1), which converts free long-chain FA into fatty acyl-CoA esters, was only marginally downregulated by the HF diet; it was not affected by HF + F. It was strongly upregulated by both TZDs, with the most pronounced effect being in HF + MSDC + F mice. This evidence concerns the gene ACSL1 and hydrops fetalis.