We detected accumulations of PF-Pink1 protein of approximately 52 kD in all three cell lines expressing Fbxo7 or its PD familial mutants when compared to the control cells expressing an empty plasmid by Western blot analyses (Figure 1), regardless of whether the cells were treated with carbonyl cyanide 3 chlorophenylhydrazone (CCCP) or not. This evidence concerns the gene PINK1 and Parkinson disease.