Transcriptome analysis demonstrated a significant increase in chemokines such as IL6 and CXCL8, along with the CXCL8 receptors, CXCR1 and CXCR2. Additionally, immune cell phenotyping via RNA-Seq and the genomic cellular analysis tool xCell provided evidence for a linear increase in Th1, Th2, Tγδ, and pro–B cell populations in EAC compared with precancerous histologies (BE, LGD, HGD). This evidence concerns the gene CXCL8 and Barrett esophagus.