TGFB1 and pulmonary fibrosis: The pseudotime-associated genes for the early and mid phases of Lineage 1 exhibited enrichment for profibrotic pathways, including lung fibrosis (P = 0.036), ECM-receptor interactions (P = 8.18 × 10–8), focal adhesion formation (P = 3.4 × 10–6), TGF-β signaling pathways (P = 0.05327), and matrix metalloproteinases (P = 0.01727) (Figure 9F, Supplemental Figure 14, and Supplemental Table 4).