To further explore the molecular signaling mechanism by which Coicis Semen attenuated ischemic-induced BBB injury and promoted angiogenesis, we measured the expression of members of the TGF-β1/ALK1/Smad1/5 signaling pathway, a key intracellular signaling system that plays a pivotal role in the proliferation of the endothelium and regulation of angiogenesis in brain recovery following ischemic stroke. Here, SMAD1 is linked to ischemic stroke.