NT5C1A and cancer: Moreover, western blot analysis of total cell lysates from cancer cells and PSCs exposed to gemcitabine (10 μM) for 48 h revealed no change in the expression of hENT1, CDA, DCK, NT5C1A, and DCTD as compared to non-exposed cells (Figure S3), suggesting that gemcitabine did not affect the expression of these enzymes in either cell type studied.