Further, we observed that this Pacrgl gene was significantly downregulated in the deficient hippocampus and significantly upregulated in the deficient liver (Figure 5B), an observation that is consistent with previous studies that noted the hippocampus and liver exhibit opposite genetic responses to short-term folate deficiency (45–48). Here, PACRGL is linked to hyperinsulinemic hypoglycemia, familial, 4.