IDH1 and anaplastic astrocytoma: On the other hand, the diffuse glioma cohort comprised samples with H3F3A, IDH1/2, and/or pTERT gene alterations and included five diffuse midline gliomas H3F3A K27M-mutant, four anaplastic oligodendrogliomas IDH-mutant and 1p/19q-codeleted, three anaplastic astrocytomas IDH-mutant, four glioblastomas IDH-mutant, and 24 glioblastomas IDH-wildtype.