In conclusion, we demonstrated that HPV16 E6/E7 proteins downregulated the expression of p-PTEN in lung cancer cells, the knockdown of PTEN further decreased the expression of TXNIP, the inhibition of TXNIP further promoted the accumulation of HIF-1α by inhibiting the translocation of nuclear HIF-1α to the cytoplasm, and consequently upregulated the expression of GLUT1 at the protein and mRNA levels. This evidence concerns the gene TXNIP and lung cancer.