To determine LINC01089’s biological functions in NSCLC cells, we transfected A549 and H1299 cells with LINC01089 small interfering RNA (siRNA) targeting LINC01089 or LINC01089 plasmid (pcDNA3.1-LINC01089), respectively, and then analyzed the efficiency of knockdown and overexpression of LINC01089 using qRT-PCR at 48 h after transfection (Figure S2A, Figure S2B). This evidence concerns the gene LINC01089 and non-small cell lung carcinoma.