Li et al. (2017) explained that miR-3619-5p promoted cell cycle arrest in prostate cancer cells. Coincidentally, our study showed that NEAT1 was a sponge of miR-3619-5p. MiR-3619-5p was revealed that its overexpression repressed cell proliferation, migration and invasion, and promoted cell cycle arrest and apoptosis in RB. These data were consistent with previous results. Besides, miR-3619-5p expression was downregulated in RB tissues and cells. Collectively, all data suggested that NEAT1 promoted RB development by sponging miR-3619-5p. This evidence concerns the gene NEAT1 and retinoblastoma.