To further understand the dual activity of Pic during EAEC infection, we first investigated the role of Pic-induced mucus secretion and mucus availability on EAEC growth by incubating EAEC with conditioned media from LS174T cells treated with Pic (degraded mucins) and LS174T cells treated with PicS258I (undegraded mucins). The gene discussed is SLC25A3; the disease is infection.