As shown in Figure 8D, the mRNAexpression levels of the inflammatory factors (IL-1β1 and CCL-2) and fibrosis related genes (TGF-β1,COL1A2, and COL3A1) were significantly increased in NASH mice and significantly decreased in YBT treated mice. This evidence concerns the gene CCL2 and metabolic dysfunction-associated steatohepatitis.