Interestingly, we noticed that the expression of NOX4 on the fifth day after ICH decreased slightly, though it still showed a significant upregulation compared to the Sham group; This is consistent with our results obtained from using immunofluorescence to evaluate the blocking efficiency of NOX4 in specific cells: NOX4 siRNA cannot effectively block NOX4 expression in microglia, while the continuous migration and activation of microglia maintain the sustained expression of NOX4 in the hematoma region. The gene discussed is NOX4; the disease is hematoma.