KP29 (H3.3K27M+/−) lung carcinoma lost the copy of chromosome 11 (Supplementary Fig. S2e) carrying the wild-type Trp53 allele, resulting in a Trp53−/− tumour, while K1327 (Trp53+/+) lung carcinoma acquired an R62Q mutation in the p53 transactivation domain, which is critical for apoptotic signalling (Supplementary Fig. S2f)33. Here, TP53 is linked to neoplasm.