XBP1 and metabolic dysfunction-associated steatotic liver disease: To determine whether direct perturbation of the 12-h clock via Xbp1 ablation promotes the consequent NAFLD phenotype, we sampled two biological replicates of mouse liver tissues at a high temporal resolution starting at circadian time 0 (CT0) and proceeding every two hours for four complete 12-h cycles (48 h in total, two complete circadian cycles), and used RNA-Sequencing (RNA-Seq) to profile their liver transcriptomes (Supplementary Figs. 3, 4, and Supplementary Data 1 and Supplementary Data 2).