Serafino et al. used a multiparametric analysis of IHC expression and subcellular localization of Wnt/β-catenin upstream (e.g., β-catenin, E-cadherin) and downstream signaling components (e.g., C-Myc, cyclin D1) in an animal model (rats) of chemically-induced CRC and human samples obtained from patients with inflammatory bowel diseases (IBD) or at sequential stages of sporadic CRC. The gene discussed is CCND1; the disease is colorectal carcinoma.