UBC and amyotrophic lateral sclerosis: In addition, to support the pathogenic role of genes already known to be associated with ALS (i.e., CHMP2B, MAPT, DYNC1H1, ERBB4, GRN, OPTN, SQSTM1, TBK1, TUBA4A, VCP, and VEGFA), our WES-based network analysis identified new potential causal genes, including RPS27A, UBA52, UBC, and UBB that were identified as the most significant bottleneck proteins connecting different complexes or pathways in the network (Figure 3a).