To further address the role of Aldob in the regulation of Akt phosphorylation in HCC, we generated stable Aldob-overexpressing liver cancer cell lines Huh7 and LM3 and found that ectopic expression of Aldob resulted in a marked reduction of Akt, GSK3β, and S6K phosphorylation levels (Fig 2C). This evidence concerns the gene AKT1 and hepatocellular carcinoma.