By comparing the positive expression of Netrin-1, cerebral infarction volume, mNSS, sticker removal test time, cell apoptosis rate, positive expression of DCC, and positive expression of ERK in three kinds of rats, the regulation of Netrin-1 in ischemic stroke and its influence on the DCC/ERK signaling pathway were comprehensively evaluated. This evidence concerns the gene NTN1 and ischemic stroke.