Quantitative real-time PCR analyses of EMT genes TWIST1, Snail1, Snail2 and CDH2 in monocultured and ASCs co-cultured MCF-7 (Figure 3G) and MDA-MB-231 (Figure 3H) revealed that most of these EMT signature genes were downregulated in co-cultured breast cancer cells. This evidence concerns the gene TWIST1 and breast cancer.