As expected, after A20.GL tumor challenge and CAR T cell treatment, CARneg CD8+ T cells in m1928z-CD40L CAR T cell-treated mice produced more IFNγ after ex vivo phorbol 12-myristate 13-acetate (PMA)/ionomycin stimulation (Supplementary Fig. 4A, B). The gene discussed is IFNG; the disease is neoplasm.