We confirmed the Lon-overexpressing B16/F10 cells in the xenograft mouse model and found that Lon overexpression shows an increasing volume of tumor mass and level in PD-L1, but not in the control cells and the Lon-overexpressing cells treated with PD-L1 blocker (figure 6B, C), suggesting that Lon overexpression in cancer cells promotes tumorigenicity in a PD-L1–dependent manner in vivo. This evidence concerns the gene CD274 and cancer.