In other clinical studies, adoptive transfer of purified antigen-specific FOXP3+Treg cells in pre-sensitized mice suppressed AHR, eosinophil recruitment, and Th2 cytokine release through the release of IL-10 and TGF-β, while the depletion of CD25+ Tregs before an allergen challenge shifted Th2 cytokine upregulation, IgE levels, eosinophilia, and AHR in allergy-resistant mice (C3H strain), concluded that Treg control disease resolution [99, 100, 119]. This evidence concerns the gene TGFB1 and Increased total eosinophil count.