Similarly, by utilizing the proximity ligation assay, Sahlberg S. with co-authors demonstrated that phosphorylated forms of Akt and DNA-PKcs also interact with each other in IR-exposed colon cancer cell lines, whereas Akt deficiency significantly impaired the rejoining of radiation-induced DNA double strand breaks and sensitized colon cancer cells to IR [36]. This evidence concerns the gene PRKDC and malignant colon neoplasm.