In addition to increased levels of cleaved caspase-3 and PARP in cancer cells treated with Dox in presence of AKT inhibitor, we also observed the increased numbers of apoptotic (e.g., Annexin V-positive cells) cells in RD rhabdomyosarcoma and GIST T-1R cells treated with combination of Dox and MK-2206 when compared to non-treated cells or cells treated with Dox alone (Figure S4), thereby revealing that AKT inhibition effectively sensitized STS and GIST cells to Dox treatment and induced apoptotic cell death. The gene discussed is CASP3; the disease is cancer.