PPARG and malignant colon neoplasm: However, our results showed that the helix H3 mutations of PPARγ LBD found in colon cancers, including PPARγ C285Y, Q286P, R288H and S289C mutations, exhibited lower binding affinities for endogenous ligands such as 15d-PGJ2 and 13S-HODE [35,36,44,45] (Figure 5F).