The CRISPRa-TF gRNA lentiviral library (named CRISPRa screen TF [CAS-TF]) was transduced at a multiplicity of infection (MOI) of 0.2 and at 550-fold coverage of the library to ensure that most cells activated a single TF and to account for the stochastic and often inefficient nature of in vitro cell differentiations (Figure 1A). The gene discussed is TF; the disease is infection.