Using this optimized protocol, we assessed the gene expression, induction and activity of important intestinal CYP enzymes in hiPSC-derived HIOs and compared these with the commonly used human colonic adenocarcinoma cell line Caco-2 and the human primary IEC-based EpiIntestinal model, closely resembling human small intestinal tissue (Ayehunie et al. 2018). The gene discussed is PPIG; the disease is colon adenocarcinoma.