Furthermore, Lee et al. (2018), performed an RNA-seq analysis on larval zebrafish after exposure to VPA and found transcriptional changes in ASD associated genes like adsl, mdbs, tsclb, and shank3 as well as differential expression in 24 other candidate autism risk genes highlighting the use of valproic acid as a model to further investigate other mechanisms of autism. Here, SHANK3 is linked to autism.