To further confirm that FOXO3a-miRNA-VEGF-A/NRP1 signaling is dysregulated in human breast cancer, expression levels of FOXO3a, miR-29b-2, miR-338, VEGF-A, and NRP1 were quantified in total RNA derived from 34 breast cancer tissues and 20 normal breast tissues. This evidence concerns the gene NRP1 and breast cancer.