The analysis of the randomly selected sample of the Her2-positive tumors treated with Dox-loaded H2.1MS1 spheres indicated the considerably reduced proliferative activity of tumor cells; lower expression of the proliferation marker Ki-67 was observed in this sample when compared with that in the Dox-loaded MS1- and saline-treated samples (Additional file 14: Figure S11). This evidence concerns the gene ERBB2 and neoplasm.