For this purpose, we prepared tumor tissue lysate, as a source of TAAs, from GBC cases (Stage I, II and IIIA) and performed immunodepletion of HSA and IgG to enrich low abundant proteins and reduce non-specific reactivity due to anti-human IgG that was used as a secondary antibody in 1D and 2-D immunoblotting experiments. The gene discussed is ALB; the disease is neoplasm.