Additional evidence came from the short hairpin RNA (shRNA) screen in a MLL-AF9 mouse model of AML [113] that identified CK1α and β-catenin as the top hits required for survival of murine AML leukemic stem cells (LSCs) and AML disease propagation in the sub lethally-irradiated recipient mice. This evidence concerns the gene CSNK1A1 and acute myeloid leukemia.