To explore the mechanisms underlying the lipid accumulation and hepatic steatosis, we assessed changes in mRNA expression of crucial genes related to lipid metabolism, focusing on fatty acids (FA) uptake (Cd36) (Figure 5B), lipogenesis (Acaca, Fasn, Scd1) (Figure 5C), FA oxidation (Cpt1a, Pparα, Acacb, Ppargc1α) (Figure 5D), and lipid secretion (Mttp, Apob) (Figure 5E) in mice liver. This evidence concerns the gene ACACA and fatty liver disease.