Mice fed with an high-saturated fat, high-fructose, and high-cholesterol (FFC) diet, as previously described [25,35,36], showed significantly increased body weight, from the 2nd week of feeding for wild type (WT) and 3rd week for high human soluble endoglin mice (hsEng) (Figure 1A), which resulted in obesity (significantly increased body weight gain (~29%)) at the end of the 24th week (Figure 1B) when compared to mice fed with a chow diet. Here, ENG is linked to obesity due to melanocortin 4 receptor deficiency.