In order to determine whether the anti-proliferative activity of isoquercitrin against SK-MEL-2 melanoma cells involves apoptotic cell death, we examined the nuclear morphology through the Hoechst staining assay, DNA fragmentation through the agarose gel electrophoresis method, and the number of cells undergoing apoptosis via the Annexin V/PI staining technique. The gene discussed is ANXA5; the disease is melanoma.