Similarly, subgroup analysis by age indicated significantly higher levels of SHMT1 methylation in ischemic stroke patients than in controls in both the evaluated age groups (age ≤60 years, ischemic stroke vs. controls: 59.46 ± 17.94% vs. 43.49 ± 20.63%, p < 0.001; age > 60 years, ischemic stroke vs. controls: 57.73 ± 16.47% vs. 41.90 ± 21.02%, p < 0.001, Figure 2). Here, SHMT1 is linked to ischemic stroke.