MICU1 and cardiac hypertrophy: Considering that mice models of cardiac hypertrophy were complex, neonatal murine ventricular myocytes (NMVMs) were isolated from left ventricular in mice hearts (Supplementary Figure 2A), infected with specific MICU1 siRNA (Figure 3A, 3B), and used the well-established NMVMs models of hypertrophy to define the specific role of MICU1 in cardiomyocytes.