No loss of heterozygosity was detected in six tumors tested and second hits in TINF2 were excluded in four of the six tumors analyzed by whole-exome sequencing (F3:III-1; Astrocytoma, F2:II-1; Melanoma and breast cancer, F1:II-4; colorectal cancer (CRC), see also Figure 1—figure supplement 2). This evidence concerns the gene TINF2 and astrocytoma (excluding glioblastoma).