In another study in which ApoE*3-Leiden mice were fed a special high-fat and cholesterol diet (15% cacao butter, 0.5% cholate, 1% cholesterol, 40.5% sucrose, 10% corn starch, 1% corn oil, and 4.7% cellulose), extensive atherosclerosis was observed [124]. This evidence concerns the gene APOE and atherosclerosis.