The paw model provided two advantages over the tongue model: 1) the HSC-3 paw model was used in our previous study to test the effect of EDNRB re-expression on proliferation, so our results were directly comparable to the those of the previous study; 2) the paw volume could be measured throughout the experiment to track cancer growth, whereas repeated measurements were not possible with the tongue cancer model without sedating the mouse. This evidence concerns the gene EDNRB and tongue cancer.