TERT and melanoma: Wild type and mutant TERT promoter fragments were inserted into pGL3Basic promoter probe vector in front of the luciferase coding sequence, and their activity was tested in two human (MCF-7, breast cancer; UACC-257, melanoma) and two rodent cell lines (D12, rat hepatocarcinoma; NIH 3T3, mouse fibroblast) by transient transfection experiments.