We noticed that the anti-melanoma efficacy of wild-type adenovirus type 5 (Ad5.WT) was much higher than ZD55 which was the viral vector of ZD55-IL-24 and was identical to Ad5.WT with the exception of E1B 55-kDa gene deletion (Supplementary Figs. 7B and 8A–E), indicating that the E1B 55-kDa gene played an important role in B16-bearing immunocompetent mouse model. Here, IL24 is linked to melanoma.