Consistently, many genes coding for key glycolytic enzymes such as GLUT1, HK2, PFKFB3, PFKP, and PKM2 were significantly upregulated in HCC192Low tumors compared to HCC192High tumors (Fig. 1e) but showed negligible alteration in their non-tumor tissues (Supplementary Fig. S1b). This evidence concerns the gene HK1 and neoplasm.