Since PSA has been widely used as a PCa screening test, and age and PCa family history are risk factors for cancer, we compared the diagnostic performance of pre-biopsy PSA, PSA at 4 ng/mL cutoff value (commonly used cutoff for further testing in PCa screening) (PSA-4), age, and PCa family history (FH) with the 25-Gene Panel urine test (25-Gene) in patients from the combination cohort who had PSA test result or family history information. This evidence concerns the gene KLK3 and cancer.