To assess the impact of increased βPix/COOL-1 rim mRNA expression and elucidate the role βPix/COOL-1 plays in this region of the tumour, we examined the effect of βPix/COOL-1 knockdown on the invasive capacity of two human GBM cell lines, U87R [36] and GBM6 [37]. The gene discussed is ARHGEF7; the disease is neoplasm.