Therefore, silica nanomaterials modified with specific recognition molecules have shown a wide range of application prospects of pathogen detection, nano-drug loading, tumor treatment, biomarkers, etc. In our study, we choose carboxyl modified silica microspheres as the carrier and coupled the antibody CPAC3 on it to realize the enrichment of CPA toxin in a food sample, using the FITC-labeled CPAN to amplify the detection signal. This evidence concerns the gene CPA1 and neoplasm.