ANXA5 and breast cancer: To further examine the impact of WYE-354 on apoptosis, BC cells were treated with different concentrations of the inhibitor (1 μM, 3 μM) for 48 h and subsequently analyzed by flow cytometry for apoptotic (via Annexin-V staining) and necrotic (via 7-aminoactinomycin D (7-AAD) staining) cells.