During iCCA tumorigenesis of the rat model, the intensity of PD-L1 expression was also increased on the 27th week (Figure 1B), indicating that PD-L1 expression is critical in iCCA tumorigenesis, and that the experimental model is suitable for studying the immune response in iCCA using the immune-checkpoint DNA vaccine. The gene discussed is CD274; the disease is infantile convulsions and choreoathetosis.