Based on our successful robust knockdown of marker genes such as firefly luciferase, mCherry in HEK293T cells, and EML4-ALK in H3122 and H2228 lung cancer cells, we speculated that this approach could be used to knock down other transgenes or genes of interest (or RNA of interest, e.g., COVID-19 RNA or noncoding RNA) that affect the biological functions of cells. Here, EML4 is linked to lung cancer.